Directed by Dr. Vincent Young, Dr. Patrick Schloss and Dr. Thomas Schmidt, The Microbiome Core serves the specific needs of investigators who are studying the role that complex microbial communities can play in shaping the overall metabolic state of their host; including, provide analytical tools to investigators to permit determination of the structure of the microbiome in mouse models of disease and assistance in the cultivation of microbes that will permit hypothesis testing in murine models including the germfree animals that are available in the Animal Care Core.
The MSMBL sequencing group offers a number of tools to aid in microbiome research. These services include DNA extraction, preparation of genomic and 16S rDNA libraries and sequence generation using the Illumina MiSeq platform. The lab has multiple Eppendorf EpMotion 5075 Liquid Handling robots that are capable of high-throughput processing of samples from DNA extraction to library prep. The Qiagen MagAttract PowerMicrobiome kit (previously MoBio PowerMag Microbiome) is our preferred DNA extraction kit as it yields high quality DNA from stool, gut and biosolids. The V4 region of the 16S rRNA-encoding gene is amplified from extracted DNA using the barcoded dual-index primers developed by Kozich et al.