DNA Isolation

The MSMBL sequencing group offers a number of tools to aid in microbiome research.  These services include DNA extraction, preparation of genomic and 16S rDNA libraries and sequence generation using the Illumina MiSeq platform. The lab has multiple Eppendorf EpMotion 5075 Liquid Handling robots that are capable of high-throughput processing of samples from DNA extraction to library prep.  The Qiagen MagAttract PowerMicrobiome kit (previously MoBio PowerMag Microbiome) is our preferred DNA extraction kit as it yields high quality DNA from stool, gut and biosolids. The V4 region of the 16S rRNA-encoding gene is amplified from extracted DNA using the barcoded dual-index primers developed by Kozich et al.

Please find our standard list of prices and services described in the table below. When you are ready to proceed, please follow the steps to submit a Request for Service.